Single particle assay of coronavirus membrane fusion with proteinaceous receptor-embedded supported bilayers
Identifieur interne : 001F05 ( Main/Exploration ); précédent : 001F04; suivant : 001F06Single particle assay of coronavirus membrane fusion with proteinaceous receptor-embedded supported bilayers
Auteurs : Deirdre A. Costello [États-Unis] ; Jean K. Millet [États-Unis] ; Chih-Yun Hsia [États-Unis] ; Gary R. Whittaker [États-Unis] ; Susan Daniel [États-Unis]Source :
- Biomaterials [ 0142-9612 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Chats, Coronavirus félin (métabolisme), Cricetinae, Fusion membranaire (physiologie), Humains, Lignée cellulaire, Membrane cellulaire (métabolisme), Membrane cellulaire (virologie), Microfluidique, Protéines de l'enveloppe virale (métabolisme), Protéines membranaires (métabolisme), Récepteurs viraux (métabolisme), Techniques d'analyse microfluidique, Traitement d'image par ordinateur.
- MESH :
- métabolisme : Coronavirus félin, Membrane cellulaire, Protéines de l'enveloppe virale, Protéines membranaires, Récepteurs viraux.
- physiologie : Fusion membranaire.
- virologie : Membrane cellulaire.
- Animaux, Chats, Cricetinae, Humains, Lignée cellulaire, Microfluidique, Techniques d'analyse microfluidique, Traitement d'image par ordinateur.
English descriptors
- KwdEn :
- Animals, CD13 Antigens (metabolism), Cats, Cell Line, Cell Membrane (metabolism), Cell Membrane (virology), Coronavirus, Feline (metabolism), Cricetinae, Humans, Image Processing, Computer-Assisted, Membrane Fusion (physiology), Membrane Proteins (metabolism), Microfluidic Analytical Techniques, Microfluidics, Receptors, Virus (metabolism), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , metabolism : CD13 Antigens, Membrane Proteins, Receptors, Virus, Viral Envelope Proteins.
- metabolism : Cell Membrane, Coronavirus, Feline.
- physiology : Membrane Fusion.
- virology : Cell Membrane.
- Animals, Cats, Cell Line, Cricetinae, Humans, Image Processing, Computer-Assisted, Microfluidic Analytical Techniques, Microfluidics.
Abstract
Total internal reflection microscopy combined with microfluidics and supported bilayers is a powerful, single particle tracking (SPT) platform for host-pathogen membrane fusion studies. But one major inadequacy of this platform has been capturing the complexity of the cell membrane, including membrane proteins. Because of this, viruses requiring proteinaceous receptors, or other unknown cellular co-factors, have been precluded from study. Here we describe a general method to integrate proteinaceous receptors and cellular components into supported bilayers for SPT fusion studies. This method is general to any enveloped virus-host cell pair, but demonstrated here for feline coronavirus (FCoV). Supported bilayers are formed from mammalian cell membrane vesicles that express feline aminopeptidase N (the viral receptor) using a cell blebbing technique. SPT is then used to identify fusion intermediates and measure membrane fusion kinetics for FCoV. Overall, the fusion results recapitulate what is observed
Url:
DOI: 10.1016/j.biomaterials.2013.06.034
PubMed: 23886734
PubMed Central: 7111216
Affiliations:
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Costello</name><affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><affiliation wicri:level="4"><nlm:aff id="aff2">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Hsia, Chih Yun" sort="Hsia, Chih Yun" uniqKey="Hsia C" first="Chih-Yun" last="Hsia">Chih-Yun Hsia</name><affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name><affiliation wicri:level="4"><nlm:aff id="aff2">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><affiliation wicri:level="4"><nlm:aff id="aff1">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author></analytic><series><title level="j">Biomaterials</title><idno type="ISSN">0142-9612</idno><idno type="eISSN">1878-5905</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>CD13 Antigens (metabolism)</term><term>Cats</term><term>Cell Line</term><term>Cell Membrane (metabolism)</term><term>Cell Membrane (virology)</term><term>Coronavirus, Feline (metabolism)</term><term>Cricetinae</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>Membrane Fusion (physiology)</term><term>Membrane Proteins (metabolism)</term><term>Microfluidic Analytical Techniques</term><term>Microfluidics</term><term>Receptors, Virus (metabolism)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chats</term><term>Coronavirus félin (métabolisme)</term><term>Cricetinae</term><term>Fusion membranaire (physiologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Membrane cellulaire (métabolisme)</term><term>Membrane cellulaire (virologie)</term><term>Microfluidique</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines membranaires (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Techniques d'analyse microfluidique</term><term>Traitement d'image par ordinateur</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>CD13 Antigens</term><term>Membrane Proteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Coronavirus, Feline</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Coronavirus félin</term><term>Membrane cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Protéines membranaires</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fusion membranaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Membrane Fusion</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Membrane cellulaire</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Membrane</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line</term><term>Cricetinae</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>Microfluidic Analytical Techniques</term><term>Microfluidics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chats</term><term>Cricetinae</term><term>Humains</term><term>Lignée cellulaire</term><term>Microfluidique</term><term>Techniques d'analyse microfluidique</term><term>Traitement d'image par ordinateur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Total internal reflection microscopy combined with microfluidics and supported bilayers is a powerful, single particle tracking (SPT) platform for host-pathogen membrane fusion studies. But one major inadequacy of this platform has been capturing the complexity of the cell membrane, including membrane proteins. Because of this, viruses requiring proteinaceous receptors, or other unknown cellular co-factors, have been precluded from study. Here we describe a general method to integrate proteinaceous receptors and cellular components into supported bilayers for SPT fusion studies. This method is general to any enveloped virus-host cell pair, but demonstrated here for feline coronavirus (FCoV). Supported bilayers are formed from mammalian cell membrane vesicles that express feline aminopeptidase N (the viral receptor) using a cell blebbing technique. SPT is then used to identify fusion intermediates and measure membrane fusion kinetics for FCoV. Overall, the fusion results recapitulate what is observed <italic>in vivo</italic>, that coronavirus entry requires binding to specific receptors, a low-pH environment, and that membrane fusion is receptor- and protease-dependent. But this method also provides quantitative kinetic rate parameters for intermediate steps in the coronavirus fusion pathway, which to our knowledge have not been obtained before. Moreover, the platform offers versatile, precise control over the sequence of triggers for fusion; these triggers may define the fusion pathway, tissue tropism, and pathogenicity of coronaviruses. Systematically varying these triggers in this platform provides a new route to study how viruses rapidly adapt to other hosts, and to identify factors that led to the emergence of zoonotic viruses, such as human SARS-CoV and the newly emerging human MERS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bolles, M" uniqKey="Bolles M">M. Bolles</name></author><author><name sortKey="Donaldson, E" uniqKey="Donaldson E">E. Donaldson</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R. Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H. Mou</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="Dekkers, D H W" uniqKey="Dekkers D">D.H.W. Dekkers</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name></author><author><name sortKey="Baker, S C" uniqKey="Baker S">S.C. Baker</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Brown, C S" uniqKey="Brown C">C.S. Brown</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Axelrod, D" uniqKey="Axelrod D">D. Axelrod</name></author><author><name sortKey="Burghardt, T P" uniqKey="Burghardt T">T.P. Burghardt</name></author><author><name sortKey="Thompson, N L" uniqKey="Thompson N">N.L. Thompson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Floyd, D L" uniqKey="Floyd D">D.L. Floyd</name></author><author><name sortKey="Ragain, J R" uniqKey="Ragain J">J.R. Ragain</name></author><author><name sortKey="Skehel, J J" uniqKey="Skehel J">J.J. Skehel</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author><author><name sortKey="Van Oijen, A M" uniqKey="Van Oijen A">A.M. van Oijen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ivanovic, T" uniqKey="Ivanovic T">T. Ivanovic</name></author><author><name sortKey="Rozendaal, R" uniqKey="Rozendaal R">R. Rozendaal</name></author><author><name sortKey="Floyd, D L" uniqKey="Floyd D">D.L. Floyd</name></author><author><name sortKey="Popvic, M" uniqKey="Popvic M">M. Popvic</name></author><author><name sortKey="Van Oijen, A M" uniqKey="Van Oijen A">A.M. Van Oijen</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Costello, D A" uniqKey="Costello D">D.A. Costello</name></author><author><name sortKey="Lee, D W" uniqKey="Lee D">D.W. Lee</name></author><author><name sortKey="Drewes, J" uniqKey="Drewes J">J. Drewes</name></author><author><name sortKey="Vasquez, K A" uniqKey="Vasquez K">K.A. Vasquez</name></author><author><name sortKey="Kisler, K" uniqKey="Kisler K">K. Kisler</name></author><author><name sortKey="Weisner, U" uniqKey="Weisner U">U. Weisner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wessels, L" uniqKey="Wessels L">L. Wessels</name></author><author><name sortKey="Elting, M W" uniqKey="Elting M">M.W. Elting</name></author><author><name sortKey="Scimeca, D" uniqKey="Scimeca D">D. Scimeca</name></author><author><name sortKey="Weninger, K" uniqKey="Weninger K">K. Weninger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matos, P M" uniqKey="Matos P">P.M. Matos</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Ahn, B" uniqKey="Ahn B">B. Ahn</name></author><author><name sortKey="Lam, W" uniqKey="Lam W">W. Lam</name></author><author><name sortKey="Santos, N C" uniqKey="Santos N">N.C. Santos</name></author><author><name sortKey="Melikyan, G B" uniqKey="Melikyan G">G.B. Melikyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Graham, R L" uniqKey="Graham R">R.L. Graham</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tresnan, D B" uniqKey="Tresnan D">D.B. Tresnan</name></author><author><name sortKey="Levis, R" uniqKey="Levis R">R. Levis</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tusell, S M" uniqKey="Tusell S">S.M. Tusell</name></author><author><name sortKey="Schittone, S A" uniqKey="Schittone S">S.A. Schittone</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeager, C L" uniqKey="Yeager C">C.L. Yeager</name></author><author><name sortKey="Ashmun, R A" uniqKey="Ashmun R">R.A. Ashmun</name></author><author><name sortKey="Williams, R K" uniqKey="Williams R">R.K. Williams</name></author><author><name sortKey="Cardellichio, C B" uniqKey="Cardellichio C">C.B. Cardellichio</name></author><author><name sortKey="Shapiro, L H" uniqKey="Shapiro L">L.H. Shapiro</name></author><author><name sortKey="Look, A T" uniqKey="Look A">A.T. Look</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scott, R E" uniqKey="Scott R">R.E. Scott</name></author><author><name sortKey="Perkins, R G" uniqKey="Perkins R">R.G. Perkins</name></author><author><name sortKey="Zschunke, M A" uniqKey="Zschunke M">M.A. Zschunke</name></author><author><name sortKey="Hoerl, B J" uniqKey="Hoerl B">B.J. Hoerl</name></author><author><name sortKey="Maercklein, P B" uniqKey="Maercklein P">P.B. Maercklein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holowka, D" uniqKey="Holowka D">D. Holowka</name></author><author><name sortKey="Baird, B" uniqKey="Baird B">B. Baird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baumgart, T" uniqKey="Baumgart T">T. Baumgart</name></author><author><name sortKey="Hammond, A T" uniqKey="Hammond A">A.T. Hammond</name></author><author><name sortKey="Sengupta, P" uniqKey="Sengupta P">P. Sengupta</name></author><author><name sortKey="Hess, S T" uniqKey="Hess S">S.T. Hess</name></author><author><name sortKey="Holowka, D A" uniqKey="Holowka D">D.A. Holowka</name></author><author><name sortKey="Baird, B A" uniqKey="Baird B">B.A. Baird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sezgin, E" uniqKey="Sezgin E">E. Sezgin</name></author><author><name sortKey="Kaiser, H J" uniqKey="Kaiser H">H.-J. Kaiser</name></author><author><name sortKey="Baumgart, T" uniqKey="Baumgart T">T. Baumgart</name></author><author><name sortKey="Schwille, P" uniqKey="Schwille P">P. Schwille</name></author><author><name sortKey="Simons, K" uniqKey="Simons K">K. Simons</name></author><author><name sortKey="Levental, I" uniqKey="Levental I">I. Levental</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Costello, D A" uniqKey="Costello D">D.A. Costello</name></author><author><name sortKey="Hsia, C Y" uniqKey="Hsia C">C.-Y. Hsia</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Porri, T" uniqKey="Porri T">T. Porri</name></author><author><name sortKey="Daniel, S" uniqKey="Daniel S">S. Daniel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="White, J M" uniqKey="White J">J.M. White</name></author><author><name sortKey="Delos, S E" uniqKey="Delos S">S.E. Delos</name></author><author><name sortKey="Brecher, M" uniqKey="Brecher M">M. Brecher</name></author><author><name sortKey="Schornberg, K" uniqKey="Schornberg K">K. Schornberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Van Der Zee, R" uniqKey="Van Der Zee R">R. van der Zee</name></author><author><name sortKey="De Haan, C A" uniqKey="De Haan C">C.A. de Haan</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Licitra, B N" uniqKey="Licitra B">B.N. Licitra</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Madu, I G" uniqKey="Madu I">I.G. Madu</name></author><author><name sortKey="Roth, S L" uniqKey="Roth S">S.L. Roth</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsuyama, S" uniqKey="Matsuyama S">S. Matsuyama</name></author><author><name sortKey="Ujike, M" uniqKey="Ujike M">M. Ujike</name></author><author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S. Morikawa</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M. Tashiro</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Regan, A D" uniqKey="Regan A">A.D. Regan</name></author><author><name sortKey="Shraybman, R" uniqKey="Shraybman R">R. Shraybman</name></author><author><name sortKey="Cohen, R D" uniqKey="Cohen R">R.D. Cohen</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sobo, K" uniqKey="Sobo K">K. Sobo</name></author><author><name sortKey="Chevallier, J" uniqKey="Chevallier J">J. Chevallier</name></author><author><name sortKey="Parton, R G" uniqKey="Parton R">R.G. Parton</name></author><author><name sortKey="Gruenberg, J" uniqKey="Gruenberg J">J. Gruenberg</name></author><author><name sortKey="Van Der Goot, F G" uniqKey="Van Der Goot F">F.G. van der Goot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hoekstra, D" uniqKey="Hoekstra D">D. Hoekstra</name></author><author><name sortKey="De Boer, T" uniqKey="De Boer T">T. de Boer</name></author><author><name sortKey="Klappe, K" uniqKey="Klappe K">K. Klappe</name></author><author><name sortKey="Wilschut, J" uniqKey="Wilschut J">J. Wilschut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Loyter, A" uniqKey="Loyter A">A. Loyter</name></author><author><name sortKey="Citovsky, V B R" uniqKey="Citovsky V">V.B.R. Citovsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Soumpasis, D M" uniqKey="Soumpasis D">D.M. Soumpasis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hohdatsu, T" uniqKey="Hohdatsu T">T. Hohdatsu</name></author><author><name sortKey="Izumiya, Y" uniqKey="Izumiya Y">Y. Izumiya</name></author><author><name sortKey="Yokoyama, Y" uniqKey="Yokoyama Y">Y. Yokoyama</name></author><author><name sortKey="Kida, K" uniqKey="Kida K">K. Kida</name></author><author><name sortKey="Koyama, H" uniqKey="Koyama H">H. Koyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taylor, A" uniqKey="Taylor A">A. Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reguera, J" uniqKey="Reguera J">J. Reguera</name></author><author><name sortKey="Santiago, C" uniqKey="Santiago C">C. Santiago</name></author><author><name sortKey="Mudgal, G" uniqKey="Mudgal G">G. Mudgal</name></author><author><name sortKey="Ordo O, D" uniqKey="Ordo O D">D. Ordoño</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author><author><name sortKey="Casasnovas, J M" uniqKey="Casasnovas J">J.M. Casasnovas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kominami, E" uniqKey="Kominami E">E. Kominami</name></author><author><name sortKey="Ueno, T" uniqKey="Ueno T">T. Ueno</name></author><author><name sortKey="Muno, D" uniqKey="Muno D">D. Muno</name></author><author><name sortKey="Katunuma, N" uniqKey="Katunuma N">N. Katunuma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, I C" uniqKey="Huang I">I.C. Huang</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Lee, K H" uniqKey="Lee K">K.H. Lee</name></author><author><name sortKey="Ghiran, S" uniqKey="Ghiran S">S. Ghiran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, Z" uniqKey="Qiu Z">Z. Qiu</name></author><author><name sortKey="Hingley, S T" uniqKey="Hingley S">S.T. Hingley</name></author><author><name sortKey="Simmons, G" uniqKey="Simmons G">G. Simmons</name></author><author><name sortKey="Yu, C" uniqKey="Yu C">C. Yu</name></author><author><name sortKey="Das Sarma, J" uniqKey="Das Sarma J">J. Das Sarma</name></author><author><name sortKey="Bates, P" uniqKey="Bates P">P. Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simmons, G" uniqKey="Simmons G">G. Simmons</name></author><author><name sortKey="Rennekamp, A J" uniqKey="Rennekamp A">A.J. Rennekamp</name></author><author><name sortKey="Bates, P" uniqKey="Bates P">P. Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Bartelink, W" uniqKey="Bartelink W">W. Bartelink</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rawlings, N D" uniqKey="Rawlings N">N.D. Rawlings</name></author><author><name sortKey="Barrett, A J" uniqKey="Barrett A">A.J. Barrett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Chu, V C" uniqKey="Chu V">V.C. Chu</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Floyd, D L" uniqKey="Floyd D">D.L. Floyd</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author><author><name sortKey="Van Oijen, A M" uniqKey="Van Oijen A">A.M. van Oijen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brian, A A" uniqKey="Brian A">A.A. Brian</name></author><author><name sortKey="Mcconnell, H M" uniqKey="Mcconnell H">H.M. McConnell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, T" uniqKey="Yang T">T. Yang</name></author><author><name sortKey="Jung, S Y" uniqKey="Jung S">S.-Y. Jung</name></author><author><name sortKey="Mao, H" uniqKey="Mao H">H. Mao</name></author><author><name sortKey="Cremer, P S" uniqKey="Cremer P">P.S. Cremer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stegmann, T" uniqKey="Stegmann T">T. Stegmann</name></author><author><name sortKey="White, J M" uniqKey="White J">J.M. White</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A. Helenius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blumenthal, R" uniqKey="Blumenthal R">R. Blumenthal</name></author><author><name sortKey="Schoch, C" uniqKey="Schoch C">C. Schoch</name></author><author><name sortKey="Puri, A" uniqKey="Puri A">A. Puri</name></author><author><name sortKey="Clague, M J" uniqKey="Clague M">M.J. Clague</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Costello, Deirdre A" sort="Costello, Deirdre A" uniqKey="Costello D" first="Deirdre A." last="Costello">Deirdre A. Costello</name></region><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><name sortKey="Hsia, Chih Yun" sort="Hsia, Chih Yun" uniqKey="Hsia C" first="Chih-Yun" last="Hsia">Chih-Yun Hsia</name><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. 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